project X

hari ni and semalam aku pulun extract DNA E.coli isolates utk project aku...

aku extract je dulu, nanti baru run PCR...

semalam dapat extract 24 isolates
tadi dapat extract 18 je, sbb semalam sempat subcultured 18 isolates je..

tadi ku abiskan sub lagi 25 isolates on blood agar... so isnin nanti leh la extract balance 25 isolates tu...

last month sebelum aku gi elective, aku dah run PCR utk 16 isolates, turned out 15/16 were positive..

so, i have another 70 isolates to run PCR...
congak2 lah sendiri, every run, max aku leh run 8 isolates... every run takes me at least 2 days if no problem, or 3-4 days if ada problem (e.g need repeating etc)...............

owhhh sempat kah?? Ku nak anta few isolates utk sequence lagi utk jadi +ve control....

eh lupa plak nak mention, all those 70 + 16 isolates tu nak buat phenotypic tests (antibiotic discs tests) plak....

hmm.. best kan?? banyak rupanye keje aku nih...

bawak bertenang je lah ye.....

moga-moga sume nye berjalan lancar.. amiiinnnnn


---