sungguh puas hati.. my hard work from 8.30am was paid off...
fuhh sapa tak sengih kalu 1st time buat PCR - menjadik and positive..
hahahha tulah citer best aku hari nih...
dari kul 8.30am aku dok lab biomol tuh...
from DNA extraction...
to mengira nak tuka micromol (umol) to pmol... (pening wooo)
to pipetting the primers, MasterMix etc...
to running the PCR... (errr that was by machine lah)...
then run on gel electrophoresis...
and the BEST when read under UV light.. wallahhh... sample 1,2,5 & 6 POSITIVE... (err.. actually 1&2 was the same sample, and 5&6 sample sama gaks).. i did 'em in duplicates (1 was old sample & 1 was newly 'sub' sample)...
yang penting -- there were 2 out of 3 ESBLs strains were CTX-M type...
hmm.. best best...
nanti aku upload gamba gel kat sini...
saje nak share 'happy' aku ngan sume
BUT... i have 403 strains to do PCR.. tapi takpe, pas dapat this +ve results, aku terus rase semangat.. cam esok nak buat lagi PCR.. hehehhe
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2 comments:
aku sememang nye tak paham.. hehehe.. tp still seronok baca yg kau happy buat lab experiment..
aku tau DNA, UV light, umol, pmol jer.
PCR n yg lelain tu mende ek?
hehehe Nana..
sebab tu aku tak explain details sgt cos nanti makin tak paham heheh..
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